Plasmid
Gal4::EX-R

Part:BBa_K3271011:Design

Designed by: Setti Belhouari   Group: iGEM19_uOttawa   (2019-10-14)


Gal4::EX-RFP-SP pSB1K3


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found in sequence at 2290
    Illegal suffix found in sequence at 3381
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 2290
    Illegal SpeI site found at 3382
    Illegal PstI site found at 3396
    Illegal NotI site found at 2296
    Illegal NotI site found at 3389
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 2290
    Illegal XhoI site found at 1026
    Illegal XhoI site found at 2052
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found in sequence at 2290
    Illegal suffix found in sequence at 3382
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 2290
    Illegal XbaI site found at 2305
    Illegal SpeI site found at 3382
    Illegal PstI site found at 3396
    Illegal AgeI site found at 3092
    Illegal AgeI site found at 3204
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Please note the upstream and downstream Gal4 locus homologies are found outside the prefix and suffix, not within the multiple cloning site. This contribution consists of an updated pSB1K3 BioBrick backbone that makes the BioBrick plasmids compatible with cloning in both E. coli and S. cerevisiae.


Source

The Gal4 upstream and downstream homologies are comprised of 100 bp each from the promoter region and the terminator region of the Gal4 locus of S. cerevisiae, respectively. The RFP cassette is identical in sequence to BBa_J04450.

References