Plasmid
Gal4::EX-R
Part:BBa_K3271011:Design
Designed by: Setti Belhouari Group: iGEM19_uOttawa (2019-10-14)
Gal4::EX-RFP-SP pSB1K3
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal prefix found in sequence at 2290
Illegal suffix found in sequence at 3381 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 2290
Illegal SpeI site found at 3382
Illegal PstI site found at 3396
Illegal NotI site found at 2296
Illegal NotI site found at 3389 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 2290
Illegal XhoI site found at 1026
Illegal XhoI site found at 2052 - 23INCOMPATIBLE WITH RFC[23]Illegal prefix found in sequence at 2290
Illegal suffix found in sequence at 3382 - 25INCOMPATIBLE WITH RFC[25]Illegal prefix found in sequence at 2290
Illegal XbaI site found at 2305
Illegal SpeI site found at 3382
Illegal PstI site found at 3396
Illegal AgeI site found at 3092
Illegal AgeI site found at 3204 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
Please note the upstream and downstream Gal4 locus homologies are found outside the prefix and suffix, not within the multiple cloning site. This contribution consists of an updated pSB1K3 BioBrick backbone that makes the BioBrick plasmids compatible with cloning in both E. coli and S. cerevisiae.
Source
The Gal4 upstream and downstream homologies are comprised of 100 bp each from the promoter region and the terminator region of the Gal4 locus of S. cerevisiae, respectively. The RFP cassette is identical in sequence to BBa_J04450.